|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21163||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Lentiviral ; reprogramming
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer EF1a_for (Common Sequencing Primers)
After lentivirus infection, Nanog and Lin28 are coexpressed by IRES2 intron. Infection with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSIN4-EF2-N2L was a gift from James Thomson (Addgene plasmid # 21163 ; http://n2t.net/addgene:21163 ; RRID:Addgene_21163)
For your References section:Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077