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(Plasmid #24603)


Item Catalog # Description Quantity Price (USD)
Plasmid 24603 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6777
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Oct4 KLF4 Sox2 c-Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (not destroyed)
  • 3′ cloning site Pme I (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The pSin4-EF2-IRES-Pur was obtained from Addgene (James Thomson's laboratory) in the form of pSin-EF2-Oct4-Pur. The WPRE/LoxP LTR portion was obtained from Addgene in the form of pWPT-GFP (Luigi Naldini's laboratory). Transgene ORFs were cloned from human factor cDNAs.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Steven T. Suhr, MSU Cellular Reprogramming Laboratory

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OKSIM was a gift from Jose Cibelli (Addgene plasmid # 24603 ; ; RRID:Addgene_24603)
  • For your References section:

    Human Induced Pluripotent Stem Cells Produced Under Xeno-Free Conditions. Ross PJ, Suhr S, Rodriguez RM, Chang EA, Wang K, Siripattarapravat K, Ko T, Cibelli JB. Stem Cells Dev. 2010 Aug;19(8):1221-9 10.1089/scd.2009.0459 PubMed 20030562