|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21162||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeLentiviral ; reprogramming
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EF1a_for (Common Sequencing Primers)
After lentivirus infection, Oct4 and Sox2 are coexpressed by EF2 promoter and IRES intron. Infection with this lentivirus, when used with pSIN4-EF2-N2L and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSIN4-EF2-O2S was a gift from James Thomson (Addgene plasmid # 21162 ; http://n2t.net/addgene:21162 ; RRID:Addgene_21162)
For your References section:Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077