193,450 results

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCB301-2μ-HDV

Plasmid

#196329

Purpose

A yeast-Escherichia coli-Agrobacterium shuttle vector

Depositor

Zhenghe Li

Article

Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

Type

Empty backbone

Expression

Plant

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

Plasmid

#172318

Purpose

CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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EC67908

Plasmid

#211795

Purpose

Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas12a, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF0675

Plasmid

#141659

Purpose

Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM16 (PRDM16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Plasmid

#209296

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-RUNX3

Plasmid

#139812

Purpose

doxycycline-inducible expression of Human RUNX3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

RUNX3 (RUNX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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TSHR-Tango

Plasmid

#66518

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TSHR (TSHR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Hygro (656-4)

Plasmid

#17446

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Lentiviral Prep

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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