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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa

Plasmid

#109525

Purpose

Muscle specific zebrafish expression of mKate2 fused to RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_TOMM20_sfCherry2(11)

Plasmid

#83033

Purpose

Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

TOMM20_sfCherry+11

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Olig2 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_ARPC1B

Plasmid

#111681

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARPC1B (ARPC1B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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XE239 Fz1-ER

Plasmid

#16816

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER (Fzd1 Rat)

Use

Xenopus expression

Available Since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
pPGKPhiC31obpA

Plasmid

#13795

Purpose

Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

PhiC31o

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pHSV-ELGA1

Plasmid

#246926

Purpose

Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity

Depositor

Teresa Alonso

Article

Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100

Insert

ELGA1 (Endo-lysosome GFP-Aequorin)

Use

Herpes viral vector

Expression

Mammalian

Mutation

D117A, D119A, D163A in aequorin gene

Promoter

IE4/5 promoter (HSV)

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHSV-ELGA

Plasmid

#246796

Purpose

Bioluminescent endolysosomal Ca2+ indicator

Depositor

Teresa Alonso

Article

Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100

Insert

ELGA (Endo-lysosome GFP-Aequorin)

Use

Herpes viral vector

Expression

Mammalian

Mutation

D119A in aequorin gene

Promoter

IE4/5 promoter (from HSV)

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_GCaMP6f (AAV1)

Viral Prep

#213859-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV_BiVIPe4_GCaMP6f (#213859). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the VIP interneuron-targeting enhancer E4. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only
Bxb1-GA_AIO-GZV-SynZiFTR_ETV2_Down-Tandem

Plasmid

#241394

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ETV2; ZF10-NS3-p65 (ETV2, RELA, KRAS Human)

Expression

Mammalian

Promoter

ZF10; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NFIX

Plasmid

#207192

Purpose

doxycycline-inducible expression of Human NFIX in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFIX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pRP-BRG1-HA-SmBiT

Plasmid

#241815

Purpose

Expresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

BRG1 (SMARCA4 Human)

Tags

HA and SmBiT

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-PFE3-P2A-GFP

Plasmid

#236180

Purpose

AAV version of Tet inducible PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-P2A-GFP

Use

AAV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVSe

Plasmid

#234896

Purpose

All-in-one plant virus expression vector for virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX)

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVEe

Plasmid

#234928

Purpose

All-in-one plant virus expression vector for virus-induced genome editing (VIGE).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

Plasmid

#236239

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.1 (CACNA1A Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3235

Plasmid

#234107

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3233

Plasmid

#234105

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

YukonOFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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