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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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AiP12109 - pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2109)

Plasmid

#208157

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDPK1

Plasmid

#23801

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDPK1 (PDPK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Anti-His [IPI-HAP1/5.13.22] (Recombinant Antibody)

Antibody

#218109-rAb

Purpose

Anti-His tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds selectively to the C-terminal HHHHHHHHHH sequence.

Depositor

Institute for Protein Innovation (IPI)

Article

Institute for Protein et al Unpublished

Recommended Applications

Western Blot

Reactivity

Synthetic

Source Species

Rabbit

Isotype

IgG

Trial Size

Not available to purchase

Available Since

May 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits
pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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RMCE halo-flag DNA-PKcs

Plasmid

#233918

Purpose

Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs (PRKDC Human)

Tags

flag-halo

Expression

Mammalian

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pKO403_lacZ_Sp

Plasmid

#174726

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-BRCA2

Plasmid

#99395

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA2 (BRCA2 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pORI28

Plasmid

#71595

Purpose

EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Type

Empty backbone

Use

Integration vector for bacteria

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-NFIA

Plasmid

#141403

Purpose

Inducible Expression of NFIA

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

NFIA (NFIA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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PEmaxNG-_2star_IVT

Plasmid

#227678

Purpose

plasmid for SpCas9-NG-PEmax** mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

NG-PEmax_2_star

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP

Plasmid

#181979

Purpose

Human RUNX1A gene overexpression

Depositor

Jan-Henning Klusmann

Article

Gialesaki et al Blood. 2023 Mar 9;141(10):1105-1118. doi

Insert

RUNX1A (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-EGFP-PARP1

Plasmid

#176146

Purpose

EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV2-CMV-eGFP

Plasmid

#230930

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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460 p3E-2A-mCherrypA

Plasmid

#195970

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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th-P2A-EGFP donor

Plasmid

#65562

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-EGFP (th Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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