225,568 results

1. pDONR221_HDLBP

   Plasmid

   #154448

   Purpose

   For recombination of HDLBP

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   HDLBP (HDLBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

   Plasmid

   #198071

   Purpose

   The AAV vector for iRFP713(V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(V256C)
   

   Use

   AAV

   Tags

   P2A-EGFP

   Expression

   Mutation

   V256C mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaster1

   Plasmid

   #58525

   Purpose

   Reprogram somatic cells to iPS cells

   Depositor

   Mario Capecchi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

   Inserts

   neo
   tk
   oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 )
   myc (MYC Human)
   EBNA-1
   Amp
   Lin28 (LIN28A Human)
   Nanog (NANOG Human)
   IRES
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAG and EF1a

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Foxd3-pIRES2 eGFP

   Plasmid

   #37269

   Depositor

   Patricia Labosky

   Insert

   Foxd3 (Foxd3 Mouse)
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pMuLE ENTR CMV Renilla Luciferase L5-L2

   Plasmid

   #62186

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   Renilla luciferase
   

   Use

   Luciferase; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmirGLO-3'UTR mouse Il6

   Plasmid

   #207126

   Purpose

   Luciferase vector containing 3'UTR for mouse Il6

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   Interleukin 6 (Il6) (Il6 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL11-GCaMP6f bb100

   Plasmid

   #158983

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23100 (sigma 70)

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-NPY [L115/13R] (Recombinant Antibody)

   Antibody

   #193439-rAb

   Purpose

   Anti-NPY (Human) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunocytochemistry

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Not available to purchase

   Available since

   April 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

9. tdTomato-N1

   Plasmid

   #54642

   Purpose

   Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Roger Tsien, Nathan Shaner, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA1775_pPBCAG-rtTAM2-IH

    Plasmid

    #124167

    Purpose

    piggyBac-based expression of rtTA-M2

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    rtTAM2-IRES-Hph
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBJ5 mCnA alpha

    Plasmid

    #17871

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    CnA alpha (Ppp3ca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-EF1a-Flpo (AAV1)

    Viral Prep

    #55637-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a

    Tags

    None

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

14. R777-E233 Hs.RASAL1

    Plasmid

    #70517

    Purpose

    Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL1 (RASAL1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    R331H

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGHE-PdNIP2-1

    Plasmid

    #108447

    Purpose

    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

    Depositor

    Richard Bélanger

    Insert

    PdNIP2-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207862

    Purpose

    AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PHB1

    Plasmid

    #212153

    Purpose

    For PHB production in E. coli (PT7-pMB1).

    Depositor

    Kang Zhou

    Insert

    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Use

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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