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(Plasmid #131636)


Item Catalog # Description Quantity Price (USD)
Plasmid 131636 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3373
  • Total vector size (bp) 4726
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    APOBEC1 fused to YTH domain
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
    YTHDF2 (a.k.a. CAHL, DF2, HGRG8, NY-REN-2)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer BGH Rev
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene 73019; pCMV-BE1
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-APOBEC1-YTH was a gift from Kate Meyer (Addgene plasmid # 131636 ; ; RRID:Addgene_131636)
  • For your References section:

    DART-seq: an antibody-free method for global m(6)A detection. Meyer KD. Nat Methods. 2019 Sep 23. pii: 10.1038/s41592-019-0570-0. doi: 10.1038/s41592-019-0570-0. 10.1038/s41592-019-0570-0 PubMed 31548708