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Binary recombinase systems for high-resolution conditional mutagenesis.
Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
Nucleic Acids Res. 2014 Jan 9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51263hGFAP-Roxed-CreExpression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter
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51264pCAG-attBr-Cre-rc-attPComponent of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation
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51265pCAG-attBr-ZsGreen-attPBxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation
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51266pCAG-b3STOPb3-ZsGreenB3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS
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51267pCAG-Co-InCreNThe N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreC
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51268pCAG-Co-InCreCThe C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreN
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51269pCAG-loxPSTOPloxP-ZsGreenCre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen
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51270pCAG-NLS-HA-B3Expression of the site-specific recombinase B3 (mouse codon-optimized)
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51271pCAG-NLS-HA-Bxb1Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)
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51272pCAG-NLS-HA-DreComponent of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
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51273pCAG-Roxed-CreComponent of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre
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51274pCAG-roxSTOProx-ZsGreenDre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen
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51275Thy1.2-NLS_HA_Dre-P2A-BFPCo-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette
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51276Thy1.2-Roxed-CreExpression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette
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51277hGFAP-NLS-HA-Dre-P2A-BFPCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter
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51278pCAG-Golden-Gate-Esp3I-DestinationCAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I
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