PurposeExpression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51276||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Promoter Thy1.2
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer GAATCCAAGTCGGAACTCTTGGC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBased on Thy1 promoter construct (https://www.addgene.org/20736/)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Thy1.2-Roxed-Cre was a gift from Pawel Pelczar (Addgene plasmid # 51276 ; http://n2t.net/addgene:51276 ; RRID:Addgene_51276)
For your References section:Binary recombinase systems for high-resolution conditional mutagenesis. Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P. Nucleic Acids Res. 2014 Jan 9. 10.1093/nar/gkt1361 PubMed 24413561