PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51277||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Promoter hGFAP
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer AGGAGACGCATCACCTCCGCTGC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBackbone and promoter based on hGFAP-fLuc (https://www.addgene.org/40589/)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hGFAP-NLS-HA-Dre-P2A-BFP was a gift from Pawel Pelczar (Addgene plasmid # 51277 ; http://n2t.net/addgene:51277 ; RRID:Addgene_51277)
For your References section:Binary recombinase systems for high-resolution conditional mutagenesis. Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P. Nucleic Acids Res. 2014 Jan 9. 10.1093/nar/gkt1361 PubMed 24413561