Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

MBP-FUS_FL_WT Citations (16)

Originally described in: Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.
Burke KA, Janke AM, Rhine CL, Fawzi NL Mol Cell. 2015 Oct 15;60(2):231-41. doi: 10.1016/j.molcel.2015.09.006. Epub 2015 Oct 8.
PubMed Journal

Articles Citing MBP-FUS_FL_WT

Articles
SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins. Perdikari TM, Murthy AC, Ryan VH, Watters S, Naik MT, Fawzi NL. bioRxiv. 2020 Jun 10. doi: 10.1101/2020.06.09.141101. PubMed
The prion-like domain of Fused in Sarcoma is phosphorylated by multiple kinases affecting liquid- and solid-phase transitions. Owen I, Rhoads S, Yee D, Wyne H, Gery K, Hannula I, Sundrum M, Shewmaker F. Mol Biol Cell. 2020 Nov 1;31(23):2522-2536. doi: 10.1091/mbc.E20-05-0290. Epub 2020 Sep 2. PubMed
Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Babinchak WM, Dumm BK, Venus S, Boyko S, Putnam AA, Jankowsky E, Surewicz WK. Nat Commun. 2020 Nov 4;11(1):5574. doi: 10.1038/s41467-020-19211-z. PubMed
SARS-CoV-2 nucleocapsid protein phase-separates with RNA and with human hnRNPs. Perdikari TM, Murthy AC, Ryan VH, Watters S, Naik MT, Fawzi NL. EMBO J. 2020 Nov 17:e106478. doi: 10.15252/embj.2020106478. PubMed

Associated Plasmids

High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate. Freibaum BD, Messing J, Yang P, Kim HJ, Taylor JP. J Cell Biol. 2021 Mar 1;220(3). pii: 211726. doi: 10.1083/jcb.202009079. PubMed
A modular tool to query and inducibly disrupt biomolecular condensates. Hernandez-Candia CN, Pearce S, Tucker CL. Nat Commun. 2021 Mar 22;12(1):1809. doi: 10.1038/s41467-021-22096-1. PubMed
Characterization of design grammar of peptides for regulating liquid droplets and aggregates of FUS. Kamagata K, Chiba R, Kawahata I, Iwaki N, Kanbayashi S, Maeda K, Takahashi H, Hirano A, Fukunaga K, Ikeda K, Kameda T. Sci Rep. 2021 Mar 23;11(1):6643. doi: 10.1038/s41598-021-86098-1. PubMed
Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. Lin YC, Kumar MS, Ramesh N, Anderson EN, Nguyen AT, Kim B, Cheung S, McDonough JA, Skarnes WC, Lopez-Gonzalez R, Landers JE, Fawzi NL, Mackenzie IRA, Lee EB, Nickerson JA, Grunwald D, Pandey UB, Bosco DA. Nat Neurosci. 2021 Aug;24(8):1077-1088. doi: 10.1038/s41593-021-00859-9. Epub 2021 May 31. PubMed
FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation. Birsa N, Ule AM, Garone MG, Tsang B, Mattedi F, Chong PA, Humphrey J, Jarvis S, Pisiren M, Wilkins OG, Nosella ML, Devoy A, Bodo C, de la Fuente RF, Fisher EMC, Rosa A, Viero G, Forman-Kay JD, Schiavo G, Fratta P. Sci Adv. 2021 Jul 21;7(30):eabf8660. doi: 10.1126/sciadv.abf8660. Print 2021 Jul. PubMed
The oncogenic transcription factor FUS-CHOP can undergo nuclear liquid-liquid phase separation. Owen I, Yee D, Wyne H, Perdikari TM, Johnson V, Smyth J, Kortum R, Fawzi NL, Shewmaker F. J Cell Sci. 2021 Sep 1;134(17). pii: 272045. doi: 10.1242/jcs.258578. Epub 2021 Sep 3. PubMed
Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads. Murthy AC, Tang WS, Jovic N, Janke AM, Seo DH, Perdikari TM, Mittal J, Fawzi NL. Nat Struct Mol Biol. 2021 Nov;28(11):923-935. doi: 10.1038/s41594-021-00677-4. Epub 2021 Nov 10. PubMed
Rational peptide design for regulating liquid-liquid phase separation on the basis of residue-residue contact energy. Kamagata K, Ariefai M, Takahashi H, Hando A, Subekti DRG, Ikeda K, Hirano A, Kameda T. Sci Rep. 2022 Aug 12;12(1):13718. doi: 10.1038/s41598-022-17829-1. PubMed
A spatiotemporal reconstruction of the C. elegans pharyngeal cuticle reveals a structure rich in phase-separating proteins. Kamal M, Tokmakjian L, Knox J, Mastrangelo P, Ji J, Cai H, Wojciechowski JW, Hughes MP, Takacs K, Chu X, Pei J, Grolmusz V, Kotulska M, Forman-Kay JD, Roy PJ. Elife. 2022 Oct 19;11:e79396. doi: 10.7554/eLife.79396. PubMed
Fused in sarcoma undergoes cold denaturation: Implications for phase separation. Felix SS, Laurents DV, Oroz J, Cabrita EJ. Protein Sci. 2023 Jan;32(1):e4521. doi: 10.1002/pro.4521. PubMed
Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. Guo L, Mann JR, Mauna JC, Copley KE, Wang H, Rubien JD, Odeh HM, Lin J, Lee BL, Ganser L, Robinson E, Kim KM, Murthy AC, Paul T, Portz B, Gleixner AM, Diaz Z, Carey JL, Smirnov A, Padilla G, Lavorando E, Espy C, Shang Y, Huang EJ, Chesi A, Fawzi NL, Myong S, Donnelly CJ, Shorter J. bioRxiv [Preprint]. 2023 Sep 4:2023.09.04.555754. doi: 10.1101/2023.09.04.555754. PubMed
A platform to induce and mature biomolecular condensates using chemicals and light. Hernandez-Candia CN, Brady BR, Harrison E, Tucker CL. Nat Chem Biol. 2024 Jan 8. doi: 10.1038/s41589-023-01520-1. PubMed

Associated Plasmids

If you have published an article using this material, please email us at [email protected] to have your article added to this page.