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RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting.
Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY
Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
154936MCP-APEX2NLS-V5-MCP-APEX2
154938dCas13d-dsRBDBPNLS-dRfxCas13d-dsRBD-BPNLS-HA
154939dCas13d-dsRBD-APEX2TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
1549404xMS2-hTR4xMS2 stem-loop-hTR-500 bp genomic sequence

Antibodies from Article