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A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes.
Madugula V, Lu L
J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85759pMyc-N1A mammalian expression vector with a Myc-tag at C-terminus.
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85761pET30axA bacterial expression vector with a His-tag at N and C-terminus.
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86049pHA-N1A mammalian expression vector with a HA-tag at C-terminus.
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86050CD8aA mammalian expression plasmid encoding CD8a without tag.
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86051CD8a-EGFPA mammalian expression plasmid encoding CD8a with a C-terminus EGFP.
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86052CD8a-cNLS-EGFPA mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
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86054CD8a-hnRNP-M-EGFPA mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.
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86055IL2Rα-EGFPA mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.
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86056EGFP-CAAXA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
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86059CFFΔC-EGFPA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.
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86060CFFΔC-MycA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.
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86061CFF-EGFPA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.
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86062CFF-MycA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag.
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86063CD8a-f-CTS-EGFPA mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.
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86064f-CTS-EGFPA mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.
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86066GST-cystin-CTSA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.
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86067GST-PC-1-CTSA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.
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86068GST-PC-2-CTSA bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.
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86069GST-prRDH-CTSA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.
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86070GST-peripherin-CTSA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.
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86071GST-rhodopsin-CTSA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.
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86072GST-RP2A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.
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86073RP2-EGFPA mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.
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86074GST-RP2-C86Y,P95LA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.
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86075EGFP-Rab8a-wtA mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.
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86076EGFP-Rab8a-QLA mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.
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86077EGFP-Rab8a-TNA mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.
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86078GST-Rab8a-wtA bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.
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86079GST-Rab8a-QLA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.
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86080GST-Rab8a-TNA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.
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86081pDMyc-TNPO1A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.
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86082TNPO1-shRNAA lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.
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86083GL2 shRNAA lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.
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86775pEGFP×2-N1A mammalian expression vector with double EGFP at C-terminus.
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86776pEGFP×3-N1A mammalian expression vector with triple EGFP at C-terminus.
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