A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes.
Madugula V, Lu L
J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15. PubMed Article
Madugula V, Lu L
J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85759 | pMyc-N1 | A mammalian expression vector with a Myc-tag at C-terminus. |
| 85761 | pET30ax | A bacterial expression vector with a His-tag at N and C-terminus. |
| 86049 | pHA-N1 | A mammalian expression vector with a HA-tag at C-terminus. |
| 86050 | CD8a | A mammalian expression plasmid encoding CD8a without tag. |
| 86051 | CD8a-EGFP | A mammalian expression plasmid encoding CD8a with a C-terminus EGFP. |
| 86052 | CD8a-cNLS-EGFP | A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP. |
| 86054 | CD8a-hnRNP-M-EGFP | A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP. |
| 86055 | IL2Rα-EGFP | A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP. |
| 86056 | EGFP-CAAX | A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP. |
| 86059 | CFFΔC-EGFP | A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP. |
| 86060 | CFFΔC-Myc | A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag. |
| 86061 | CFF-EGFP | A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP. |
| 86062 | CFF-Myc | A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag. |
| 86063 | CD8a-f-CTS-EGFP | A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP. |
| 86064 | f-CTS-EGFP | A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP. |
| 86066 | GST-cystin-CTS | A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag. |
| 86067 | GST-PC-1-CTS | A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag. |
| 86068 | GST-PC-2-CTS | A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag. |
| 86069 | GST-prRDH-CTS | A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag. |
| 86070 | GST-peripherin-CTS | A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag. |
| 86071 | GST-rhodopsin-CTS | A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag. |
| 86072 | GST-RP2 | A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag. |
| 86073 | RP2-EGFP | A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP. |
| 86074 | GST-RP2-C86Y,P95L | A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag. |
| 86075 | EGFP-Rab8a-wt | A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP. |
| 86076 | EGFP-Rab8a-QL | A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP. |
| 86077 | EGFP-Rab8a-TN | A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP. |
| 86078 | GST-Rab8a-wt | A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST. |
| 86079 | GST-Rab8a-QL | A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST. |
| 86080 | GST-Rab8a-TN | A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST. |
| 86081 | pDMyc-TNPO1 | A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag. |
| 86082 | TNPO1-shRNA | A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection. |
| 86083 | GL2 shRNA | A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection. |
| 86775 | pEGFP×2-N1 | A mammalian expression vector with double EGFP at C-terminus. |
| 86776 | pEGFP×3-N1 | A mammalian expression vector with triple EGFP at C-terminus. |