Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases
Ioannidi EI, Yarnall MTN, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Roberts N, Zhang L, Vakulskas CA, Walker II JA, Kadina AP, Zepeda AE, Holden K, Gootenberg JS, Abudayyeh OO
bioRxiv 2021.11.01.466786 Article
Ioannidi EI, Yarnall MTN, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Roberts N, Zhang L, Vakulskas CA, Walker II JA, Kadina AP, Zepeda AE, Holden K, Gootenberg JS, Abudayyeh OO
bioRxiv 2021.11.01.466786 Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 179104 | pDY0216 PASTEv1 PE2-P2A-Bxb1 | PASTEv1 PE2-P2A-Bxb1 |
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| 179105 | pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT | pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT |
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| 179106 | pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT | pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT |
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| 179107 | pDY0207 ACTB atgRNA with v1 scaffold | ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA |
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| 179108 | pDY0663 ACTB atgRNA with v2 scaffold | ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold |
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| 179109 | pDY0209 ACTB Nicking guide +48 | ACTB N-term Nicking guide 1 +48 guide |
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| 179110 | pDY0310 TP901-1 Integrase | TP901-1 Integrase expression vector |
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| 179111 | pDY0312 PhiBT Integrase | PhiBT Integrase expression vector |
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| 179112 | pDY0750 pCAG-NLS-BceINT | BceINT expression vector |
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| 179113 | pDY0738 pCAG-NLS-SscINT | SscINT expression vector |
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| 179114 | pDY0739 pCAG-NLS-SacINT | SacINT expression vector |
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| 179115 | pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle) | Cargo EGFP with AttP Bxb1 site (Parent minicircle) |
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| 179116 | pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle) | Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle) |
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