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Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases
Ioannidi EI, Yarnall MTN, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Roberts N, Zhang L, Vakulskas CA, Walker II JA, Kadina AP, Zepeda AE, Holden K, Gootenberg JS, Abudayyeh OO
bioRxiv 2021.11.01.466786
Article

Plasmids from Article

ID Plasmid Purpose  
179104pDY0216 PASTEv1 PE2-P2A-Bxb1PASTEv1 PE2-P2A-Bxb1
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179105pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
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179106pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
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179107pDY0207 ACTB atgRNA with v1 scaffoldACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA
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179108pDY0663 ACTB atgRNA with v2 scaffoldACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold
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179109pDY0209 ACTB Nicking guide +48ACTB N-term Nicking guide 1 +48 guide
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179110pDY0310 TP901-1 IntegraseTP901-1 Integrase expression vector
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179111pDY0312 PhiBT IntegrasePhiBT Integrase expression vector
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179112pDY0750 pCAG-NLS-BceINTBceINT expression vector
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179113pDY0738 pCAG-NLS-SscINTSscINT expression vector
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179114pDY0739 pCAG-NLS-SacINTSacINT expression vector
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179115pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)Cargo EGFP with AttP Bxb1 site (Parent minicircle)
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179116pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
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Recombinant Antibodies from Article