Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ
Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed Article
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ
Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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40255 | pcDNA3-AKAR2-CR |
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40256 | pcDNA3-Camui-CR |
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40257 | pcDNA3.1/Puro-CAG-VSFP-CR |
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40258 | pCAGGS-Raichu-RhoA-CR |
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40259 | pcDNA3-Clover | GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap |
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40260 | pcDNA3-mRuby2 | RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap |
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54551 | Clover-Lifeact-7 | Localization: Actin, Excitation: 505, Emission: 515 |
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54561 | mRuby2-NT-C1 | Localization: C1 Cloning Vector, Excitation: 559, Emission: 600 |
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54575 | Clover-pBAD | Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016. |
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54612 | mRuby2-NT-N1 | Localization: N1 Cloning Vector, Excitation: 559, Emission: 600 |
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54614 | mRuby2-N1 | Localization: N1 Cloning Vector, Excitation: 559, Emission: 600 |
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54674 | mRuby2-Lifeact-7 | Localization: Actin, Excitation: 559, Emission: 600 |
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54768 | mRuby2-C1 | Localization: C1 Cloning Vector, Excitation: 559, Emission: 600 |
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54771 | mRuby2-pBAD | Localization: Protein Expression Vector , Excitation: 559, Emission: 600 |
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55888 | mRuby2-Actin-7 | Localization: Actin, Excitation: 559, Emission: 600 |
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55889 | mRuby2-Actin-C-18 | Localization: Actin, Excitation: 559, Emission: 600 |
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55890 | mRuby2-Alpha-Actinin-19 | Localization: Alpha Actinin, Excitation: 559, Emission: 600 |
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55891 | mRuby2-Calnexin-N-14 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
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55892 | mRuby2-Calreticulin-N-16 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
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55893 | mRuby2-CENPB-N-22 | Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600 |
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55894 | mRuby2-Cx43-7 | Localization: Gap Junctions, Excitation: 559, Emission: 600 |
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55895 | mRuby2-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
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55896 | mRuby2-Farnesyl-5 | Localization: Membrane, Excitation: 559, Emission: 600 |
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55897 | mRuby2-H2B-6 | Localization: Nucleus/Histones, Excitation: 559, Emission: 600 |
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55898 | mRuby2-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 559, Emission: 600 |
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55899 | mRuby2-JUP-C-14 | Localization: Tight Junctions, Excitation: 559, Emission: 600 |
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55900 | mRuby2-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600 |
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55901 | mRuby2-LaminA-C-18 | Localization: Nuclear Envelope, Excitation: 559, Emission: 600 |
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55902 | mRuby2-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 559, Emission: 600 |
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55903 | mRuby2-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600 |
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55904 | mRuby2-MAPTau-C-10 | Localization: Microtubules, Excitation: 559, Emission: 600 |
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55905 | mRuby2-Mito-7 | Localization: Mitochondria, Excitation: 559, Emission: 600 |
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55906 | mRuby2-MyosinIIA-C-18 | Localization: Cytoskeleton, Excitation: 559, Emission: 600 |
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55907 | mRuby2-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 559, Emission: 600 |
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55908 | mRuby2-Nup50-N-10 | Localization: Nuclear Pore, Excitation: 559, Emission: 600 |
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55909 | mRuby2-PDHA1-N-10 | Localization: Mitochondria, Excitation: 559, Emission: 600 |
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55910 | mRuby2-PMP-N-10 | Localization: Peroxisomes, Excitation: 559, Emission: 600 |
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55911 | mRuby2-Rab5a-7 | Localization: Ras GTPase, Excitation: 559, Emission: 600 |
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55912 | mRuby2-SiT-N-15 | Localization: Golgi TGN, Excitation: 559, Emission: 600 |
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55913 | mRuby2-TOMM20-N-10 | Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600 |
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55914 | mRuby2-Tubulin-6 | Localization: Microtubules, Excitation: 559, Emission: 600 |
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55915 | mRuby2-Tubulin-C-18 | Localization: Microtubules, Excitation: 559, Emission: 600 |
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55916 | mRuby2-ZO1-C-14 | Localization: Tight Junctions, Excitation: 559, Emission: 600 |
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55917 | mRuby2-Zyxin-6 | Localization: Focal Adhesions, Excitation: 559, Emission: 600 |
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56301 | Clover-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515 |
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56302 | Clover-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 505, Emission: 515 |
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56303 | Clover-Nup50-N-10 | Localization: Nuclear Pore, Excitation: 505, Emission: 515 |
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56304 | Clover-PDHA1-N-10 | Localization: Mitochondria, Excitation: 505, Emission: 515 |
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56305 | Clover-PMP-N-10 | Localization: Peroxisomes, Excitation: 505, Emission: 515 |
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56306 | Clover-SiT-N-15 | Localization: Golgi TGN, Excitation: 505, Emission: 515 |
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56307 | Clover-TOMM20-N-10 | Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515 |
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56370 | Clover-LaminA-C-18 | Localization: Nuclear Envelope, Excitation: 505, Emission: 515 |
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56371 | Clover-MAPTau-C-10 | Localization: Microtubules, Excitation: 505, Emission: 515 |
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56372 | Clover-MyosinIIA-C-18 | Localization: Cytoskeleton, Excitation: 505, Emission: 515 |
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56373 | Clover-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 505, Emission: 515 |
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56374 | Clover-Tubulin-C-18 | Localization: Microtubules, Excitation: 505, Emission: 515 |
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56375 | Clover-ZO1-C-14 | Localization: Tight Junctions, Excitation: 505, Emission: 515 |
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56522 | Clover-Cx43-7 | Localization: Gap Junctions, Excitation: 505, Emission: 515 |
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56528 | Clover-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 505, Emission: 515 |
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56529 | Clover-Mito-7 | Localization: Mitochondria, Excitation: 505, Emission: 515 |
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56530 | Clover-Rab5a-7 | Localization: Ras GTPase, Excitation: 505, Emission: 515 |
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56531 | Clover-Zyxin-6 | Localization: Focal Adhesions, Excitation: 505, Emission: 515 |
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