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Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ
Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
40255pcDNA3-AKAR2-CR
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40256pcDNA3-Camui-CR
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40257pcDNA3.1/Puro-CAG-VSFP-CR
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40258pCAGGS-Raichu-RhoA-CR
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40259pcDNA3-CloverGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
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40260pcDNA3-mRuby2RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
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54551Clover-Lifeact-7Localization: Actin, Excitation: 505, Emission: 515
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54561mRuby2-NT-C1Localization: C1 Cloning Vector, Excitation: 559, Emission: 600
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54575Clover-pBADLocalization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.
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54612mRuby2-NT-N1Localization: N1 Cloning Vector, Excitation: 559, Emission: 600
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54614mRuby2-N1Localization: N1 Cloning Vector, Excitation: 559, Emission: 600
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54674mRuby2-Lifeact-7Localization: Actin, Excitation: 559, Emission: 600
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54768mRuby2-C1Localization: C1 Cloning Vector, Excitation: 559, Emission: 600
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54771mRuby2-pBADLocalization: Protein Expression Vector , Excitation: 559, Emission: 600
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55888mRuby2-Actin-7Localization: Actin, Excitation: 559, Emission: 600
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55889mRuby2-Actin-C-18Localization: Actin, Excitation: 559, Emission: 600
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55890mRuby2-Alpha-Actinin-19Localization: Alpha Actinin, Excitation: 559, Emission: 600
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55891mRuby2-Calnexin-N-14Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600
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55892mRuby2-Calreticulin-N-16Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600
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55893mRuby2-CENPB-N-22Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600
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55894mRuby2-Cx43-7Localization: Gap Junctions, Excitation: 559, Emission: 600
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55895mRuby2-CytERM-N-17Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600
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55896mRuby2-Farnesyl-5Localization: Membrane, Excitation: 559, Emission: 600
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55897mRuby2-H2B-6Localization: Nucleus/Histones, Excitation: 559, Emission: 600
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55898mRuby2-H2B-C-10Localization: Nucleus/Histones, Excitation: 559, Emission: 600
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55899mRuby2-JUP-C-14Localization: Tight Junctions, Excitation: 559, Emission: 600
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55900mRuby2-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600
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55901mRuby2-LaminA-C-18Localization: Nuclear Envelope, Excitation: 559, Emission: 600
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55902mRuby2-Lysosomes-20Localization: Lysosome Membrane, Excitation: 559, Emission: 600
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55903mRuby2-MannII-N-10Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600
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55904mRuby2-MAPTau-C-10Localization: Microtubules, Excitation: 559, Emission: 600
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55905mRuby2-Mito-7Localization: Mitochondria, Excitation: 559, Emission: 600
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55906mRuby2-MyosinIIA-C-18Localization: Cytoskeleton, Excitation: 559, Emission: 600
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55907mRuby2-Myotilin-C-14Localization: Cytoskeleton, Excitation: 559, Emission: 600
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55908mRuby2-Nup50-N-10Localization: Nuclear Pore, Excitation: 559, Emission: 600
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55909mRuby2-PDHA1-N-10Localization: Mitochondria, Excitation: 559, Emission: 600
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55910mRuby2-PMP-N-10Localization: Peroxisomes, Excitation: 559, Emission: 600
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55911mRuby2-Rab5a-7Localization: Ras GTPase, Excitation: 559, Emission: 600
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55912mRuby2-SiT-N-15Localization: Golgi TGN, Excitation: 559, Emission: 600
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55913mRuby2-TOMM20-N-10Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600
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55914mRuby2-Tubulin-6Localization: Microtubules, Excitation: 559, Emission: 600
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55915mRuby2-Tubulin-C-18Localization: Microtubules, Excitation: 559, Emission: 600
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55916mRuby2-ZO1-C-14Localization: Tight Junctions, Excitation: 559, Emission: 600
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55917mRuby2-Zyxin-6Localization: Focal Adhesions, Excitation: 559, Emission: 600
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56301Clover-CytERM-N-17Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515
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56302Clover-MannII-N-10Localization: Mannosidase/Golgi, Excitation: 505, Emission: 515
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56303Clover-Nup50-N-10Localization: Nuclear Pore, Excitation: 505, Emission: 515
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56304Clover-PDHA1-N-10Localization: Mitochondria, Excitation: 505, Emission: 515
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56305Clover-PMP-N-10Localization: Peroxisomes, Excitation: 505, Emission: 515
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56306Clover-SiT-N-15Localization: Golgi TGN, Excitation: 505, Emission: 515
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56307Clover-TOMM20-N-10Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515
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56370Clover-LaminA-C-18Localization: Nuclear Envelope, Excitation: 505, Emission: 515
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56371Clover-MAPTau-C-10Localization: Microtubules, Excitation: 505, Emission: 515
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56372Clover-MyosinIIA-C-18Localization: Cytoskeleton, Excitation: 505, Emission: 515
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56373Clover-Myotilin-C-14Localization: Cytoskeleton, Excitation: 505, Emission: 515
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56374Clover-Tubulin-C-18Localization: Microtubules, Excitation: 505, Emission: 515
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56375Clover-ZO1-C-14Localization: Tight Junctions, Excitation: 505, Emission: 515
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56522Clover-Cx43-7Localization: Gap Junctions, Excitation: 505, Emission: 515
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56528Clover-Lysosomes-20Localization: Lysosome Membrane, Excitation: 505, Emission: 515
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56529Clover-Mito-7Localization: Mitochondria, Excitation: 505, Emission: 515
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56530Clover-Rab5a-7Localization: Ras GTPase, Excitation: 505, Emission: 515
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56531Clover-Zyxin-6Localization: Focal Adhesions, Excitation: 505, Emission: 515
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