pcDNA3-mRuby2
(Plasmid #40260)

• Purpose: RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
• Depositing Lab: Michael Lin
• Publication: Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
• Citations: 47 Articles

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5478
• Total vector size (bp): 6192
• Modifications to backbone: His6 and Xpress tags at N-terminus of insert.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRuby2
• Species: Synthetic; Entacmaea quadricolor
• Insert Size (bp): 714
• Promoter: CMV IE
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • Xpress (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mRuby2 is the brightest red fluorescent protein (RFP) characterized to date, with robust performance in protein fusions. Also, it is useful as FRET acceptor for Clover GFP in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap.