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pCAGGS-Raichu-RhoA-CR
(Plasmid #40258)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40258 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4719
  • Total vector size (bp) 7050
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Raichu-RhoA-CR
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2331
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCAGGS-Raichu-RhoA was gifted by M. Matsuda (Department of Pathology and Biology of Diseases, Kyoto University, Japan).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

SEYFP was replaced with GFP Clover and SECFP was replaced with mRuby2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-Raichu-RhoA-CR was a gift from Michael Lin (Addgene plasmid # 40258 ; http://n2t.net/addgene:40258 ; RRID:Addgene_40258)
  • For your References section:

    Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245