|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40256||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5320
- Total vector size (bp) 8194
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat), Synthetic
Insert Size (bp)2874
- Promoter CMV IE
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMVFW
- 3′ sequencing primer pcDNA3.1BGHRev (Common Sequencing Primers)
Venus was replaced with GFP Clover and CFP S175G was replaced with RFP mRuby2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Camui-CR was a gift from Michael Lin (Addgene plasmid # 40256 ; http://n2t.net/addgene:40256 ; RRID:Addgene_40256)
For your References section:Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245