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pcDNA3-AKAR2-CR
(Plasmid #40255)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40255 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5489
  • Total vector size (bp) 7398
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AKAR2
  • Alt name
    forkhead associated domain 1 (FHA1) (Rad53p 22–162)
  • Species
    Synthetic
  • Insert Size (bp)
    1909
  • Promoter CMV IE
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The AKAR2 plasmid was gifted by J. Zhang (Department of Pharmacology and Molecular Sciences, John Hopkins Medicine).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

ECFP was replaced with GFP Clover and Citrine was replaced with RFP mRuby2.

Note: there is a small sequence insertion downstream of the insert that adds restriction sites. This is not present in the full plasmids sequence, so please refer to Addgene's QC sequence with the BGH-Rev primer for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-AKAR2-CR was a gift from Michael Lin (Addgene plasmid # 40255 ; http://n2t.net/addgene:40255 ; RRID:Addgene_40255)
  • For your References section:

    Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245