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pcDNA3.1-Clover-mRuby2
(Plasmid #49089)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49089 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-Clover (Addgene plasmid #40259)
  • Backbone manufacturer
    Michael Lin
  • Backbone size w/o insert (bp) 6127
  • Total vector size (bp) 6910
  • Modifications to backbone
    inserted NheI/DraIII fragment containing mRuby2 from Addgene plasmid #40260 into XbaI/DraIII site of Addgene plasmid #40259
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Clover-mRuby2 fusion
  • Species
    Synthetic; Aequorea victoria
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site DraIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Clover-mRuby2 was a gift from Kurt Beam (Addgene plasmid # 49089)