|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49089||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3-Clover (Addgene plasmid #40259)
Backbone manufacturerMichael Lin
- Backbone size w/o insert (bp) 6127
- Total vector size (bp) 6910
Modifications to backboneinserted NheI/DraIII fragment containing mRuby2 from Addgene plasmid #40260 into XbaI/DraIII site of Addgene plasmid #40259
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameClover-mRuby2 fusion
SpeciesSynthetic; Aequorea victoria
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site DraIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-Clover-mRuby2 was a gift from Kurt Beam (Addgene plasmid # 49089)
Generated by Addgene from full sequence supplied by depositor.