|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27793||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEYFP N1
- Backbone size w/o insert (bp) 3984
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemVenus N1
Insert Size (bp)720
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CCAAAATCAACGGGACTTTCC
- 3′ sequencing primer CAGGTTCAGGGGGAGGTGTGG (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
This construct was created by mutagenesis of pEYFP-N1. When compared with EYFP, Venus contains the following mutations: F46L, F64L, M153T, V163A and S175G. This construct also contains A206K mutation to create a monomeric form of the fluorescent protein.
This plasmid contains a MCS upstream of mVenus so that a gene of interest can be cloned to create a fusion protein with a C-terminal mVenus tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mVenus N1 was a gift from Steven Vogel (Addgene plasmid # 27793 ; http://n2t.net/addgene:27793 ; RRID:Addgene_27793)
For your References section:Cerulean, Venus, and VenusY67C FRET reference standards. Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS. Biophys J. 2006 Dec 15. 91(12):L99-L101. 10.1529/biophysj.106.096206 PubMed 17040988