Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3-mRuby2 Citations (29)

Plasmid Article: Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
PubMed Journal

Articles Citing pcDNA3-mRuby2

Articles
Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y, Nagai T. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23. PubMed

Associated Plasmids

Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells. Scott BL, Hoppe AD. Sci Rep. 2015 Jul 1;5:10270. doi: 10.1038/srep10270. PubMed
Developing Fast Fluorescent Protein Voltage Sensors by Optimizing FRET Interactions. Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ. PLoS One. 2015 Nov 20;10(11):e0141585. doi: 10.1371/journal.pone.0141585. eCollection 2015. PubMed

Associated Plasmids

Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis. Takeuchi H, Higashiyama T. Nature. 2016 Mar 10;531(7593):245-8. doi: 10.1038/nature17413. PubMed
FRET reveals multiple interaction states between two component signalling system proteins of M. tuberculosis. Agrawal R, Kumar V P, Ramanan H, Saini DK. Biochim Biophys Acta. 2016 Jul;1860(7):1498-507. doi: 10.1016/j.bbagen.2016.04.011. Epub 2016 Apr 19. PubMed
A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Kaberniuk AA, Shemetov AA, Verkhusha VV. Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864. PubMed

Associated Plasmids

Developmentally-regulated RNA-binding Protein 1 (Drb1)/RNA-binding Motif Protein 45 (RBM45), a Nuclear-cytoplasmic Trafficking Protein, Forms TAR DNA-binding Protein 43 (TDP-43)-mediated Cytoplasmic Aggregates. Mashiko T, Sakashita E, Kasashima K, Tominaga K, Kuroiwa K, Nozaki Y, Matsuura T, Hamamoto T, Endo H. J Biol Chem. 2016 May 12. pii: jbc.M115.712232. PubMed
Rho GTPase Rif signals through IRTKS, Eps8 and WAVE2 to generate dorsal membrane ruffles and filopodia. Sudhaharan T, Sem KP, Liew HF, Yu YH, Goh WI, Chou AM, Ahmed S. J Cell Sci. 2016 Jun 8. pii: jcs.179655. PubMed
Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin. Baker CA, Elyada YM, Parra A, Bolton MM. Elife. 2016 Aug 15;5. pii: e14193. doi: 10.7554/eLife.14193. PubMed

Associated Plasmids

Immunogenomic engineering of a plug-and-(dis)play hybridoma platform. Pogson M, Parola C, Kelton WJ, Heuberger P, Reddy ST. Nat Commun. 2016 Aug 17;7:12535. doi: 10.1038/ncomms12535. PubMed
Single Amino Acid Substitution N659D in HIV-2 Envelope Glycoprotein (Env) Impairs Viral Release and Hampers BST-2 Antagonism. Dufrasne FE, Lombard C, Goubau P, Ruelle J. Viruses. 2016 Oct 14;8(10). pii: E285. PubMed
mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PubMed

Associated Plasmids

A KCNC3 mutation causes a neurodevelopmental, non-progressive SCA13 subtype associated with dominant negative effects and aberrant EGFR trafficking. Khare S, Nick JA, Zhang Y, Galeano K, Butler B, Khoshbouei H, Rayaprolu S, Hathorn T, Ranum LPW, Smithson L, Golde TE, Paucar M, Morse R, Raff M, Simon J, Nordenskjold M, Wirdefeldt K, Rincon-Limas DE, Lewis J, Kaczmarek LK, Fernandez-Funez P, Nick HS, Waters MF. PLoS One. 2017 May 3;12(5):e0173565. doi: 10.1371/journal.pone.0173565. eCollection 2017. PubMed
Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2. Park H, Kim NY, Lee S, Kim N, Kim J, Heo WD. Nat Commun. 2017 Jun 23;8(1):30. doi: 10.1038/s41467-017-00060-2. PubMed

Associated Plasmids

Microtubule cross-linking activity of She1 ensures spindle stability for spindle positioning. Zhu Y, An X, Tomaszewski A, Hepler PK, Lee WL. J Cell Biol. 2017 Aug 9. pii: jcb.201701094. doi: 10.1083/jcb.201701094. PubMed
A common mechanism by which type 2A von Willebrand disease mutations enhance ADAMTS13 proteolysis revealed with a von Willebrand factor A2 domain FRET construct. Lynch CJ, Cawte AD, Millar CM, Rueda D, Lane DA. PLoS One. 2017 Nov 29;12(11):e0188405. doi: 10.1371/journal.pone.0188405. eCollection 2017. PubMed
A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells. Srinivasan S, Burckhardt CJ, Bhave M, Chen Z, Chen PH, Wang X, Danuser G, Schmid SL. PLoS Biol. 2018 Apr 18;16(4):e2005377. doi: 10.1371/journal.pbio.2005377. eCollection 2018 Apr. PubMed

Associated Plasmids

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ. Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4. PubMed
Tunable molecular tension sensors reveal extension-based control of vinculin loading. LaCroix AS, Lynch AD, Berginski ME, Hoffman BD. Elife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927. PubMed

Associated Plasmids

Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Paatero I, Sauteur L, Lee M, Lagendijk AK, Heutschi D, Wiesner C, Guzman C, Bieli D, Hogan BM, Affolter M, Belting HG. Nat Commun. 2018 Aug 31;9(1):3545. doi: 10.1038/s41467-018-05851-9. PubMed
Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression. Parola C, Mason DM, Zingg A, Neumeier D, Reddy ST. Methods Mol Biol. 2018;1850:79-111. doi: 10.1007/978-1-4939-8730-6_7. PubMed
Sensory perception drives food avoidance through excitatory basal forebrain circuits. Patel JM, Swanson J, Ung K, Herman A, Hanson E, Ortiz-Guzman J, Selever J, Tong Q, Arenkiel BR. Elife. 2019 May 10;8. pii: 44548. doi: 10.7554/eLife.44548. PubMed
Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Kalappurakkal JM, Anilkumar AA, Patra C, van Zanten TS, Sheetz MP, Mayor S. Cell. 2019 Jun 13;177(7):1738-1756.e23. doi: 10.1016/j.cell.2019.04.037. Epub 2019 May 16. PubMed
Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Ouchi R, Togo S, Kimura M, Shinozawa T, Koido M, Koike H, Thompson W, Karns RA, Mayhew CN, McGrath PS, McCauley HA, Zhang RR, Lewis K, Hakozaki S, Ferguson A, Saiki N, Yoneyama Y, Takeuchi I, Mabuchi Y, Akazawa C, Yoshikawa HY, Wells JM, Takebe T. Cell Metab. 2019 May 30. pii: S1550-4131(19)30247-5. doi: 10.1016/j.cmet.2019.05.007. PubMed
The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest. Duman JG, Mulherkar S, Tu YK, Erikson KC, Tzeng CP, Mavratsas VC, Ho TS, Tolias KF. Elife. 2019 Aug 28;8. pii: 47566. doi: 10.7554/eLife.47566. PubMed
Flow cytometry-based FRET identifies binding intensities in PPARgamma1 protein-protein interactions in living cells. Trumper V, von Knethen A, Preuss A, Ermilov E, Hackbarth S, Kuchler L, Gunne S, Schafer A, Bornhutter T, Vereb G, Ujlaky-Nagy L, Brune B, Roder B, Schindler M, Parnham MJ, Knape T. Theranostics. 2019 Jul 28;9(19):5444-5463. doi: 10.7150/thno.29367. eCollection 2019. PubMed
Optogenetic Control of Spine-Head JNK Reveals a Role in Dendritic Spine Regression. Hollos P, John JM, Lehtonen JV, Coffey ET. eNeuro. 2020 Feb 20;7(1). pii: ENEURO.0303-19.2019. doi: 10.1523/ENEURO.0303-19.2019. Print 2020 Jan/Feb. PubMed
Quantitative Determination of Dark Chromophore Population Explains the Apparent Low Quantum Yield of Red Fluorescent Proteins. Prangsma JC, Molenaar R, van Weeren L, Bindels DS, Haarbosch L, Stouthamer J, Gadella TWJ Jr, Subramaniam V, Vos WL, Blum C. J Phys Chem B. 2020 Feb 27;124(8):1383-1391. doi: 10.1021/acs.jpcb.9b10396. Epub 2020 Feb 17. PubMed
Upregulated PKM2 in Macrophages Exacerbates Experimental Arthritis via STAT1 Signaling. Xu J, Jiang C, Wang X, Geng M, Peng Y, Guo Y, Wang S, Li X, Tao P, Zhang F, Han Y, Ning Q, Zhu W, Meng L, Lu S. J Immunol. 2020 Jul 1;205(1):181-192. doi: 10.4049/jimmunol.1901021. Epub 2020 Jun 5. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.