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Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques.
Rimbault C, Breillat C, Compans B, Toulme E, Nunes Vicente F, Fernandez-Monreal M, Mascalchi P, Genuer C, Puente-Munoz V, Gauthereau I, Hosy E, Claverol S, Giannone G, Chamma I, Mackereth CD, Poujol C, Choquet D, Sainlos M
Elife. 2024 Jan 3;13:e69620. doi: 10.7554/eLife.69620. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135528pCAG_Xph15-eGFP-CCR5TCEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135529pCAG_Xph18-eGFP-CCR5TCEncodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135530pCAG_Xph20-eGFP-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135531pCAG_Xph20-mRuby2-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135532pCAG_Xph20-mEos3.2-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135533pCAG_Xph15-mNeonGreen-CCR5TCEncodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135534pCAG_Xph20-mNeonGreen-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135536pCAG_Xph15-SNAPf-CCR5TCEncodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
135537pCAG_Xph20-SNAPf-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain
135538pCMV_Xph15-GCaMP6fEncodes a specific PSD-95 binder (Xph15) fused to GCaMP6f
135539pCAG_Xph20-GCaMP7fEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7f
187442AAV_Syn_Xph15_eGFP_CCR5TCEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
187443AAV_Syn_Xph18_eGFP_CCR5TCEncodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
187444AAV_Syn_Xph20_eGFP_CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain
216534pCAG_Xph20-GCaMP7f-CCR5TCEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Antibodies from Article