Engineering paralog-specific PSD-95 synthetic binders as potent and minimally invasive imaging probes
Rimbault C, Breillat C, Compans B, Toulmé E, Vicente FN, Monreal MF, Mascalchi P, Genuer C, Puente-Muñoz V, Gauthereau I, Hosy E, Giannone G, Chamma I, Mackereth CD, Poujol C, Choquet D, Sainlos M
BioRxiv Article
Rimbault C, Breillat C, Compans B, Toulmé E, Vicente FN, Monreal MF, Mascalchi P, Genuer C, Puente-Muñoz V, Gauthereau I, Hosy E, Giannone G, Chamma I, Mackereth CD, Poujol C, Choquet D, Sainlos M
BioRxiv Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 135528 | pCAG_Xph15-eGFP-CCR5TC | Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135529 | pCAG_Xph18-eGFP-CCR5TC | Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135530 | pCAG_Xph20-eGFP-CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135531 | pCAG_Xph20-mRuby2-CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135532 | pCAG_Xph20-mEos3.2-CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135533 | pCAG_Xph15-mNeonGreen-CCR5TC | Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135534 | pCAG_Xph20-mNeonGreen-CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135536 | pCAG_Xph15-SNAPf-CCR5TC | Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135537 | pCAG_Xph20-SNAPf-CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain |
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| 135538 | pCMV_Xph15-GCaMP6f | Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f |
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| 135539 | pCAG_Xph20-GCaMP7f | Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f |
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| 187442 | AAV_Syn_Xph15_eGFP_CCR5TC | Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 187443 | AAV_Syn_Xph18_eGFP_CCR5TC | Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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| 187444 | AAV_Syn_Xph20_eGFP_CCR5TC | Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain |
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