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(Plasmid #175177)


Item Catalog # Description Quantity Price (USD)
Plasmid 175177 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 7116
  • Modifications to backbone
    Kozak sequence followed by GCaMP6s-GSG-P2A-mBeRFP inserted in between loxP sites in reverse reading direction, driven by CAG promoter
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Insert Size (bp)
  • Promoter CAG
  • Tag / Fusion Protein
    • P2A-mBeRFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mBeRFP sequence is based on the sequences and mutations described in Yang et al., 2013: Yang, J., Wang, L., Yang, F., Luo, H., Xu, L., Lu, J., Zeng, S., and Zhang, Z. (2013). mBeRFP, an improved large stokes shift red fluorescent protein. PLoS One 8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA was a gift from Bernardo Sabatini (Addgene plasmid # 175177 ; ; RRID:Addgene_175177)
  • For your References section:

    Bombesin-like peptide recruits disinhibitory cortical circuits and enhances fear memories. Melzer S, Newmark ER, Mizuno GO, Hyun M, Philson AC, Quiroli E, Righetti B, Gregory MR, Huang KW, Levasseur J, Tian L, Sabatini BL. Cell. 2021 Oct 28;184(22):5622-5634.e25. doi: 10.1016/j.cell.2021.09.013. Epub 2021 Oct 4. 10.1016/j.cell.2021.09.013 PubMed 34610277