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pCMV CEPIA2mt
(Plasmid #58218)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58218 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV/myc/mito
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4799
  • Total vector size (bp) 6404
  • Modifications to backbone
    Duplicate mitochondria targeting sequence.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CEPIA2mt
  • Alt name
    green fluorescent calcium-measuring organelle-entrapped protein indicator for the mitochondria
  • Species
    Synthetic
  • Insert Size (bp)
    1605
  • Mutation
    GCaMP2 N263Y E282V M339L
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtaggcgtg
  • 3′ sequencing primer tagaaggcacagtcgagg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV CEPIA2mt was a gift from Masamitsu Iino (Addgene plasmid # 58218 ; http://n2t.net/addgene:58218 ; RRID:Addgene_58218)
  • For your References section:

    Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M. Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153. 10.1038/ncomms5153 PubMed 24923787