PurposeLocalization: Actin, Excitation: 559, Emission: 600
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55889||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneACTB (a.k.a. BRWS1, PS1TP5BP1)
- Promoter CMV
/ Fusion Protein
- mRuby2 (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
. Excitation = 559; Emission = 600
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mRuby2-Actin-C-18 was a gift from Michael Davidson (Addgene plasmid # 55889 ; http://n2t.net/addgene:55889 ; RRID:Addgene_55889)
For your References section:Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245