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pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
(Plasmid #71720)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71720 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCFJ151
  • Backbone size w/o insert (bp) 7373
  • Total vector size (bp) 11395
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRANSPORT INHIBITOR RESPONSE 1
  • Alt name
    TIR1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1884
  • Mutation
    codon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated
  • Entrez Gene
    TIR1 (a.k.a. AT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1)
  • Promoter eef-1A.1 (eft-3)
  • Tag / Fusion Protein
    • mRuby (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR) was a gift from Abby Dernburg (Addgene plasmid # 71720 ; http://n2t.net/addgene:71720 ; RRID:Addgene_71720)
  • For your References section:

    The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Zhang L, Ward JD, Cheng Z, Dernburg AF. Development. 2015 Nov 9. pii: dev.129635. 10.1242/dev.129635 PubMed 26552885