Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pJW1354
(Plasmid #69490)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69490 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 3862
  • Vector type
    CRISPR ; template to generate cassettes for -mediated knock-in

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    246

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1354 was a gift from Jordan Ward (Addgene plasmid # 69490 ; http://n2t.net/addgene:69490 ; RRID:Addgene_69490)
  • For your References section:

    The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Zhang L, Ward JD, Cheng Z, Dernburg AF. Development. 2015 Nov 9. pii: dev.129635. 10.1242/dev.129635 PubMed 26552885