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pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
(Plasmid #71719)


Item Catalog # Description Quantity Price (USD)
Plasmid 71719 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7373
  • Total vector size (bp) 9593
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    auxin-responsive protein IAA17
  • Alt name
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
  • Mutation
    minimal degron sequence (71-114aa) with start codon
  • Entrez Gene
    AXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)
  • Promoter eef-1A.1 (eft-3)
  • Tag / Fusion Protein
    • EmGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR) was a gift from Abby Dernburg (Addgene plasmid # 71719 ; ; RRID:Addgene_71719)
  • For your References section:

    The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Zhang L, Ward JD, Cheng Z, Dernburg AF. Development. 2015 Nov 9. pii: dev.129635. 10.1242/dev.129635 PubMed 26552885