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A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60903pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation
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60904pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLSThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64
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60905pLV-sgCDKN1B#2 BFPA lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP
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60906pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPREThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging
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60907pHR-scFv-GCN4-sfGFP-GB1-dWPREThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging
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60909pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-PuroA minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging
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60910pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPREdCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system
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60911pcDNA4TO-H2B-SunTag24x_v1Enocdes a histone H2B fused to the SunTag for single molecule imaging
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60912pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro Enocdes Camsap1L1 fused to the SunTag for single molecule imaging
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60913pcDNA4TO-mito-mCherry-24xGCN4_v1A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry
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60914pcDNA4TO-mito-mCherry-10xGCN4_v4A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry
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61056pcDNA4TO-sfGFP-24xGCN4_v1A direct fusion of sfGFP to the 24xGCN4_v1 peptide array
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61057pcDNA4TO-24xGCN4_v1-P2A-sfGFPsfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site
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61058pcDNA4TO-24xGCN4_v4-sfGFPA direct fusion of sfGFP to the 24xGCN4_v4 peptide array
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72228pcDNA4TO-Kif18b-24xGCN4_v1A fusion of the Kif18b motor protein to the v1 SunTag peptide array
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72229pHRdSV40-K560-24xGCN4_v4A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array
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