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Addgene

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
(Plasmid #60910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Promoter dSV40 Promoter
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • HA (C terminal on insert)
    • 2x NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SV40pro-F2
  • 3′ sequencing primer mTagBFP-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE was a gift from Ron Vale (Addgene plasmid # 60910 ; http://n2t.net/addgene:60910 ; RRID:Addgene_60910)

  • For your References section:

    A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933
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