PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60903||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCas9 dead
For more information, visit http://valelab.ucsf.edu/external/research/suntag.html
Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP was a gift from Ron Vale (Addgene plasmid # 60903 ; http://n2t.net/addgene:60903 ; RRID:Addgene_60903)
For your References section:A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933