A tool kit for rapid cloning and expression of recombinant antibodies.
Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885. PubMed Article
Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50365 | pVITRO1-102.1F10-IgE/λ | Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ) |
| 50366 | pVITRO1-102.1F10-IgG1/λ | Expresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ) |
| 50367 | pVITRO1-102.1F10-IgG2/λ | Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ) |
| 50368 | pVITRO1-102.1F10-IgG3/λ | Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ) |
| 50369 | pVITRO1-102.1F10-IgG4/λ | Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ) |
| 50370 | pVITRO1-102.1F10-IgA1/λ | Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ) |
| 50371 | pVITRO1-102.1F10-IgA2/λ | Expresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ) |
| 50383 | pVITRO1-M80-F2-IgG1/κ | Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain |
| 52213 | pVITRO1-dV-IgG1/κ | Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies |
| 52214 | pVITRO1-dV-IgG1/λ | Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies |
| 61878 | pVITRO1-dV-IgE/κ | Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies |
| 61879 | pVITRO1-dV-IgE/λ | Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies |
| 61880 | pVITRO1-102.1F10-IgM/λ | Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ) |
| 61881 | pVITRO1-Trastuzumab-IgM/κ | Expresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ) |
| 61883 | pVITRO1-Trastuzumab-IgG1/κ | Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ) |
| 61884 | pVITRO1-Trastuzumab-IgG2/κ | Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ) |
| 61885 | pVITRO1-Trastuzumab-IgG3/κ | Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ) |
| 61887 | pVITRO1-Trastuzumab-IgG4/κ | Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ) |
| 61888 | pVITRO1-Trastuzumab-IgA1/κ | Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ) |
| 61889 | pVITRO1-Trastuzumab-IgA2/κ | Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ) |