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A tool kit for rapid cloning and expression of recombinant antibodies.
Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50365pVITRO1-102.1F10-IgE/λExpresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)
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50366pVITRO1-102.1F10-IgG1/λExpresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)
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50367pVITRO1-102.1F10-IgG2/λExpresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)
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50368pVITRO1-102.1F10-IgG3/λExpresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)
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50369pVITRO1-102.1F10-IgG4/λExpresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)
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50370pVITRO1-102.1F10-IgA1/λExpresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)
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50371pVITRO1-102.1F10-IgA2/λExpresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)
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50383pVITRO1-M80-F2-IgG1/κExpresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain
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52213pVITRO1-dV-IgG1/κHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
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52214pVITRO1-dV-IgG1/λHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
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61878pVITRO1-dV-IgE/κHuman immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
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61879pVITRO1-dV-IgE/λHuman immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
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61880pVITRO1-102.1F10-IgM/λExpresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
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61881pVITRO1-Trastuzumab-IgM/κExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)
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61883pVITRO1-Trastuzumab-IgG1/κExpresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)
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61884pVITRO1-Trastuzumab-IgG2/κExpresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)
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61885pVITRO1-Trastuzumab-IgG3/κExpresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)
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61887pVITRO1-Trastuzumab-IgG4/κExpresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)
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61888pVITRO1-Trastuzumab-IgA1/κExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)
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61889pVITRO1-Trastuzumab-IgA2/κExpresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)
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Recombinant Antibodies from Article