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pT18mobsacB
(Plasmid #72648)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72648 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pT18mobsacB
  • Backbone size (bp) 6500
  • Modifications to backbone
    Tetracycline resistant derivative of pK18mobsacB
  • Vector type
    sucrose counter-selection allelic exchange vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The strain grows to a lower final turbidity than other E coli strains and is cold sensitive. Do not store @ 4C.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer AACAGCTATGACATGA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hai-Lei Wei, Suma Chakravarthy, Jay N. Worley and Alan Collmer
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cell Microbiol. 2013 Apr;15(4):601-18. doi: 10.1111/cmi.12059. Epub 2012 Nov 21. Wei HL, Chakravarthy S, Worley JN, Collmer A.
Consequences of flagellin export through the type III secretion system of Pseudomonas syringae reveal a major difference in the innate immune systems of mammals and the model plant Nicotiana benthamiana.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT18mobsacB was a gift from Brian Kvitko (Addgene plasmid # 72648 ; http://n2t.net/addgene:72648 ; RRID:Addgene_72648)