pT18mobsacB
(Plasmid #72648)

• Purpose: (Empty Backbone) sucrose counter-selection allelic exchange vector for Pseudomonas syringae
• Depositing Lab: Brian Kvitko
• Publication: Kvitko lab Gateway compatible allelic exchange plasmids (unpublished)

Backbone

• Vector backbone: pT18mobsacB
• Backbone size (bp): 6500
• Modifications to backbone: Tetracycline resistant derivative of pK18mobsacB
• Vector type: sucrose counter-selection allelic exchange vector

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The strain grows to a lower final turbidity than other E coli strains and is cold sensitive. Do not store @ 4C.
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: AACAGCTATGACATGA

Resource Information

• Supplemental Documents:
  • Updated gene deletion protocol
• A portion of this plasmid was derived from a plasmid made by: Hai-Lei Wei, Suma Chakravarthy, Jay N. Worley and Alan Collmer
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Cell Microbiol. 2013 Apr;15(4):601-18. doi: 10.1111/cmi.12059. Epub 2012 Nov 21. Wei HL, Chakravarthy S, Worley JN, Collmer A.
Consequences of flagellin export through the type III secretion system of Pseudomonas syringae reveal a major difference in the innate immune systems of mammals and the model plant Nicotiana benthamiana.