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(Plasmid #177839)


Item Catalog # Description Quantity Price (USD)
Plasmid 177839 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 3282
  • Vector type
    Bacterial gene replacement
  • Selectable markers
    Counterselection: Sucrose

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Special M13R binding site, M13R: CAGGAAACAGCTATGAC
  • 3′ sequencing primer M13F: TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sequences were derived from pK18mobsacB (ATCC 87097)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence for this plasmid has been deposited with Genbank (Accession # OK423783).

Sequences were derived from pK18mobsacB (ATCC 87097), which was originally described in Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69–73. doi:10.1016/0378-1119(94)90324-7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK18msB was a gift from Christopher Johnson (Addgene plasmid # 177839 ; ; RRID:Addgene_177839)
  • For your References section:

    Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering. Ling C, Peabody GL, Salvachua D, Kim YM, Kneucker CM, Calvey CH, Monninger MA, Munoz NM, Poirier BC, Ramirez KJ, St John PC, Woodworth SP, Magnuson JK, Burnum-Johnson KE, Guss AM, Johnson CW, Beckham GT. Nat Commun. 2022 Aug 22;13(1):4925. doi: 10.1038/s41467-022-32296-y. 10.1038/s41467-022-32296-y PubMed 35995792