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pK18sB
(Plasmid #177838)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 177838 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pK18sB
  • Backbone size (bp) 3038
  • Vector type
    Bacterial gene replacement
  • Selectable markers
    Counterselection: Sucrose

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Special M13R binding site, M13R: CAGGAAACAGCTATGAC
  • 3′ sequencing primer M13F: TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sequences were derived from pK18mobsacB (ATCC 87097).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence for this plasmid has been deposited with Genbank (Accession # MH166772).

Sequences were derived from pK18mobsacB (ATCC 87097), which was originally described in Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69–73. doi:10.1016/0378-1119(94)90324-7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK18sB was a gift from Gregg Beckham & Christopher Johnson (Addgene plasmid # 177838 ; http://n2t.net/addgene:177838 ; RRID:Addgene_177838)
  • For your References section:

    Thermochemical wastewater valorization via enhanced microbial toxicity tolerance. Jayakody LN, Johnson CW, Whitham JM, Giannone RJ, Black BA, Cleveland NS, Klingeman DM, Michener WE, Olstad JL, Vardon DR, Brown RC, Brown SD, Hettich RL, Guss AM, Beckham GT. Energy Environ. Sci., 2018,11, 1625-1638 10.1039/c8ee00460a