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(Plasmid #137908)


Item Catalog # Description Quantity Price (USD)
Plasmid 137908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 5600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
  • Alt name
  • Species
    Methanosarcina mazei
  • Mutation
    Containing N346A, C348S mutations
  • Promoter pBAD

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PylRS-AS was a gift from Wenshe Liu (Addgene plasmid # 137908 ; ; RRID:Addgene_137908)
  • For your References section:

    Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader. Lee YJ, Schmidt MJ, Tharp JM, Weber A, Koenig AL, Zheng H, Gao J, Waters ML, Summerer D, Liu WR. Chem Commun (Camb). 2016 Oct 18;52(85):12606-12609. doi: 10.1039/c6cc05959g. 10.1039/c6cc05959g PubMed 27711380