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(Plasmid #127411)


Item Catalog # Description Quantity Price (USD)
Plasmid 127411 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
  • Alt name
  • Species
    Methanosarcina mazei
  • Mutation
  • Promoter araBAD

Cloning Information

  • Cloning method Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-pylT-N346A/C348A was a gift from Wenshe Liu (Addgene plasmid # 127411 ; ; RRID:Addgene_127411)
  • For your References section:

    A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum. Wang YS, Fang X, Wallace AL, Wu B, Liu WR. J Am Chem Soc. 2012 Feb 15;134(6):2950-3. doi: 10.1021/ja211972x. Epub 2012 Feb 6. 10.1021/ja211972x PubMed 22289053