pBBR1MCS-2
(Plasmid #85168)

• Purpose: (Empty Backbone) Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
• Depositing Lab: Kenneth Peterson
• Depositing Organization: Louisiana State University Health Sciences Center, Shreveport
  Located in United States of America
• Publication: Kovach et al Gene. 1995 Dec 1;166(1):175-6.
• Citations: 20 Articles

Backbone

• Vector backbone: pBBR1MCS
• Modifications to backbone: swapped antibiotic resistance cassette
• Vector type: Bacterial Expression
• Promoter: Constitutive (derepressed P-lac)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression).
MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning