Purpose(Empty Backbone) Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85168||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneswapped antibiotic resistance cassette
Vector typeBacterial Expression
- Promoter Constitutive (derepressed P-lac)
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression).
MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBBR1MCS-2 was a gift from Kenneth Peterson (Addgene plasmid # 85168 ; http://n2t.net/addgene:85168 ; RRID:Addgene_85168)
For your References section:Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, Peterson KM. Gene. 1995 Dec 1;166(1):175-6. 0378111995005841 [pii] PubMed 8529885