240,492 results

1. pGNW4

   Plasmid

   #122088

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Use

   Tags

   AID(71-114)

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and TEF1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Cas9-Blast

   Plasmid

   #83481

   Purpose

   Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

   Depositor

   Mohan Babu

   Inserts

   SpCas9
   Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   Tight Tre and human phosphoglycerate kinase 1 pro…

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG_IFITM1

   Plasmid

   #179955

   Purpose

   Expression vector for human IFITM1.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM1 (IFITM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P13A

   Promoter

   Available since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-UBXD3-Strep-HA

   Plasmid

   #113488

   Purpose

   Expression of human UBXD3 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD3 (UBXN10 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-blueAKAR

   Plasmid

   #118470

   Purpose

   cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCIDT-AMP-Bv16SrRNA

   Plasmid

   #218518

   Purpose

   pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

   Depositor

   Ami Bhatt

   Article

   Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

   Insert

   16S rRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-HA-UBE3BΔHECT-IRES-Puro

   Plasmid

   #107347

   Purpose

   Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3BΔHECT (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deletion HECT domain (757-end)

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBJ5 mCnB

   Plasmid

   #17872

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   CnB (Ppp3r1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. II701: pMVP (L1-L4) cTnT promoter

    Plasmid

    #121690

    Purpose

    pMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    cTnT promoter (Cardiomyocyte specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro-irf3-shrna1

    Plasmid

    #127648

    Purpose

    Knock-down of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 shRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCLIPf-Cox8A Control Plasmid

    Plasmid

    #101130

    Purpose

    Expression of CLIP-Tagged Cox8A in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    Cox8A (COX8A Human)
    

    Use

    Tags

    CLIP-tag (CLIPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pExp-mCherry

    Plasmid

    #112569

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1X-Nup88

    Plasmid

    #87338

    Purpose

    To express human Nup88 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP88 (NUP88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA TfR1-miniR

    Plasmid

    #181986

    Purpose

    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Parvalbumin scFv [L114/3]

    Plasmid

    #190504

    Purpose

    Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
    

    Use

    Tags

    HA, 6xHis, Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E229 Hs.RASA2

    Plasmid

    #70513

    Purpose

    Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KRas FAK biosensor

    Plasmid

    #78301

    Purpose

    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    KRas FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

20. BL21ΔF

    Bacterial Strain

    #102259

    Purpose

    ompF deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only