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Improved methods for marking active neuron populations.
Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, Trojanowski NF, Ledderose J, Zolnik TA, Das A, Patel D, Brown TA, Sachdev RNS, Eickholt BJ, Larkum ME, Turrigiano GG, Dana H, Gee CE, Oertner TG, Hope BT, Schreiter ER
Nat Commun. 2018 Oct 25;9(1):4440. doi: 10.1038/s41467-018-06935-2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101060pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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101061pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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101062pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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101063pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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101064pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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119723pAAV_hsyn_NES-his-CaMPARI2-F391W-L398VAAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
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137185pTol2-HuC(elavl3)-CaMPARI2pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)
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