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pHelper_ShCAST_sgRNA Citations (7)

Originally described in: RNA-guided DNA insertion with CRISPR-associated transposases.
Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. Epub 2019 Jun 6. PubMed Journal

Articles Citing pHelper_ShCAST_sgRNA

Articles
Multicopy chromosomal integration using CRISPR-associated transposases. Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S. ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073. PubMed

Associated Plasmids
Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S. Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi: 10.1093/nar/gkab752. PubMed

Associated Plasmids
Target site selection and remodelling by type V CRISPR-transposon systems. Querques I, Schmitz M, Oberli S, Chanez C, Jinek M. Nature. 2021 Nov;599(7885):497-502. doi: 10.1038/s41586-021-04030-z. Epub 2021 Nov 10. PubMed
Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. PubMed

Associated Plasmids
A versatile Cas12k-based genetic engineering toolkit (C12KGET) for metabolic engineering in genetic manipulation-deprived strains. Cui Y, Dong H, Tong B, Wang H, Chen X, Liu G, Zhang D. Nucleic Acids Res. 2022 Aug 26;50(15):8961-8973. doi: 10.1093/nar/gkac655. PubMed
Structural basis for the assembly of the type V CRISPR-associated transposon complex. Schmitz M, Querques I, Oberli S, Chanez C, Jinek M. Cell. 2022 Dec 22;185(26):4999-5010.e17. doi: 10.1016/j.cell.2022.11.009. Epub 2022 Nov 25. PubMed
Structures of the holo CRISPR RNA-guided transposon integration complex. Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH. Nature. 2023 Jan;613(7945):775-782. doi: 10.1038/s41586-022-05573-5. Epub 2022 Nov 28. PubMed

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