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RNA-guided DNA insertion with CRISPR-associated transposases.
Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F
Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. Epub 2019 Jun 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
127921pHelper_ShCAST_sgRNAExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).
127922pHelper_ShCASTExpresses ShCAST and a crRNA targeting PSP1
127923pHelper_AcCASTExpresses AcCAST and a crRNA targeting PSP1
127924pDonor_ShCAST_kanRKanamycin resistance donor for ShCAST
127925pDonor_AcCAST_kanRKanamycin resistance donor for AcCAST
127926pTarget_CASTTarget plasmid containing PSP1
135524pXT128_TwinStrep-SUMO-ShCas12kProtein expression construct for ShCast12k
135525pXT129_TwinStrep-SUMO-ShTnsBProtein expression construct for ShTnsB
135526pXT130_TwinStrep-SUMO-ShTnsCProtein expression construct for ShTnsC
135527pXT131_TwinStrep-SUMO-ShTniQProtein expression construct for ShTniQ

Antibodies from Article