pLV6-Bmal-luc
(Plasmid #68833)

• Purpose: Expression of Bmal1-luc in mammalian cells
• Depositing Lab: Steven Brown
• Publication: Steven Brown plasmids (unpublished)

Backbone

• Vector backbone: pLenti6
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 10234
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmal1 regulatory sequences
• Species: M. musculus (mouse)
• Insert Size (bp): 4300
• Entrez Gene: Bmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AleI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: lucNRev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry