pLV6-Bmal-luc
(Plasmid #68833)

• Purpose: Expression of Bmal1-luc in mammalian cells
• Depositing Lab: Steven Brown
• Publication: Steven Brown plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	68833	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pLenti6
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 10234
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmal1 regulatory sequences
• Species: M. musculus (mouse)
• Insert Size (bp): 4300
• Entrez Gene: Arntl (a.k.a. Ar, Arnt3, BMAL1b, Bma, Bmal1, MO, MOP3, bHLHe, bHLHe5, bmal1b')
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AleI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: lucNRev

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry
• Article Citing this Plasmid:
  • 1 Reference