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pABpuro-BluF
(Plasmid #46824)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46824 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWPI
  • Backbone manufacturer
    Addgene plasmid 12254
  • Modifications to backbone
    The EF1α promoter and gfp gene were removed from lentiviral backbone plasmid pWPI.
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmal1
  • Alt name
    Arntl
  • Alt name
    aryl hydrocarbon receptor nuclear translocator-like
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Arntl (a.k.a. Arnt3, BMAL1b, Bmal1, MOP3, bHLHe5, bmal1b')
  • Promoter Bmal1
  • Tag / Fusion Protein
    • luciferase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer LucNRev
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The insert consists of 1 kb of mouse Bmal1 upstream region and 53 nucleotides of exon 1, fused in-frame to the luciferase coding region, and followed by 1 kb of Bmal1 3′UTR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABpuro-BluF was a gift from Steven Brown (Addgene plasmid # 46824 ; http://n2t.net/addgene:46824 ; RRID:Addgene_46824)
  • For your References section:

    The period length of fibroblast circadian gene expression varies widely among human individuals. Brown SA, Fleury-Olela F, Nagoshi E, Hauser C, Juge C, Meier CA, Chicheportiche R, Dayer JM, Albrecht U, Schibler U. PLoS Biol. 2005 Oct;3(10):e338. Epub 2005 Sep 27. 10.1371/journal.pbio.0030338 PubMed 16167846