161,492 results

1. EGFP-Tau(T217E)

   Plasmid

   #187024

   Purpose

   Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

   Depositor

   George Bloom

   Article

   Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 217 to Glutamic acid

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ROCK1

   Plasmid

   #187296

   Purpose

   Express GFP ROCK1

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. synIRES_RC25

   Plasmid

   #189970

   Purpose

   Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   synIRES_RC25
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-PTEN-shRNA-3001

   Plasmid

   #25639

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-3001 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_KRAS_p.G12C

   Plasmid

   #81667

   Purpose

   Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G12C

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG-Hc∆18-AA

   Plasmid

   #86559

   Purpose

   Plasmid encodes blinded measles virus H glycoprotein

   Depositor

   Jakob Reiser

   Article

   Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

   Insert

   Measles virus H glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y463A, R515A

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito cpVenus control

   Plasmid

   #186792

   Purpose

   Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)

   Viral Prep

   #51669-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Promoter

   Syn

   Tags

   None

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pET-Sac-Abeta(M1-42)

    Plasmid

    #71875

    Purpose

    Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-42) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-Pol-IIΔ

    Plasmid

    #35176

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the entire C-terminal domain (repeats…

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221-SLC25A5_STOP

    Plasmid

    #161173

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB2F

    Plasmid

    #195289

    Purpose

    Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98928

    Purpose

    Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid

    #164576

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Eric Schmidt, Nathaniel Heintz, Alexander Nectow

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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