A Tn7-based broad-range bacterial cloning and expression system.
Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP
Nat Methods. 2005 Jun;2(6):443-8. PubMed Article
Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP
Nat Methods. 2005 Jun;2(6):443-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63120 | pUC18-mini-Tn7T-Gm-lacZ | mini-Tn7 lacZ transcriptional fusion vector |
| 63121 | pUC18T-mini-Tn7T-Gm | mini-Tn7 vector |
| 64946 | pFLP3 | Flp recombinase expression vector |
| 64948 | pFCM1 | source of CmR FRT cassette |
| 64949 | pFGM1 | source of GmR FRT cassette |
| 64950 | pFKM1 | source of KmR FRT cassette |
| 64951 | pFTC1 | source of TcR FRT cassette |
| 64952 | pFTP1 | source of TpR FRT cassette |
| 64953 | pUC18-mini-Tn7 | mini-Tn7 base vector |
| 64954 | pUC18-mini-Tn7T | mini-Tn7 base vector with transcriptional terminator |
| 64956 | pUC18PV-mini-Tn7T | pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA |
| 64957 | pUC18T-mini-Tn7T | mini-Tn7 base vector with transcriptional terminator and oriT |
| 64958 | pUC18R6K-mini-Tn7T | mini-Tn7 base vector with transcriptional terminator |
| 64961 | pUC18-mini-Tn7T-Gm-GW | mini-Tn7 Gateway destination vector |
| 64963 | pUC18-mini-Tn7T-Gm-lux | mini-Tn7 luxCDABE transcriptional fusion vector |
| 64965 | pUC18-mini-Tn7T-LAC | mini-Tn7 vector with Ptac expression cassette |
| 64966 | pUC18T-mini-Tn7T-Gm-REP | mini-Tn7 vector with oriR6K, for attTn7 site determination |
| 64967 | pTNS1 | Tn7 transposase expression |
| 64968 | pTNS2 | Tn7 transposase expression |
| 64969 | pUC18R6KT-mini-Tn7T-Km | mini-Tn7 base vector with transcriptional terminator and oriT |
| 65009 | pFRT2 | FRT casette base vector |