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pUC18-mini-Tn7T-LAC
(Plasmid #64965)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64965 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pUC18-mini-Tn7T-Gm
  • Backbone manufacturer
    Herbert Schweizer
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    also ampicillin resistant
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genbank Accession # AY599234

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-mini-Tn7T-LAC was a gift from Herbert Schweizer (Addgene plasmid # 64965 ; http://n2t.net/addgene:64965 ; RRID:Addgene_64965)
  • For your References section:

    A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923