Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pFLP3
(Plasmid #64946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64946 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFLP1
  • Backbone manufacturer
    Herbert Schweizer
  • Total vector size (bp) 10925
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flp recombinase

Resource Information

Depositor Comments

GenBank Accession # AY597273

Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLP3 was a gift from Herbert Schweizer (Addgene plasmid # 64946 ; http://n2t.net/addgene:64946 ; RRID:Addgene_64946)
  • For your References section:

    A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923