|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerHerbert Schweizer
- Total vector size (bp) 10925
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline
Copy numberHigh Copy
Gene/Insert nameFlp recombinase
GenBank Accession # AY597273
Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFLP3 was a gift from Herbert Schweizer (Addgene plasmid # 64946 ; http://n2t.net/addgene:64946 ; RRID:Addgene_64946)
For your References section:A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923