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(Plasmid #64969)


Item Catalog # Description Quantity Price (USD)
Plasmid 64969 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone manufacturer
    Herbert Schweizer
  • Modifications to backbone
    addition of FRT-flanked KmR marker
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18R6KT-mini-Tn7T-Km was a gift from Herbert Schweizer (Addgene plasmid # 64969 ; ; RRID:Addgene_64969)
  • For your References section:

    A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923