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pTNS3
(Plasmid #63127)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63127 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pTNS2
  • Modifications to backbone
    strong promoter P1 was added to drive transposase expression
  • Vector type
    Bacterial Expression
  • Promoter P1 and Ptac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    requires pir-positive E. coli for replication
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTNS3 was a gift from Herbert Schweizer (Addgene plasmid # 63127 ; http://n2t.net/addgene:63127 ; RRID:Addgene_63127)
  • For your References section:

    Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Choi KH, Mima T, Casart Y, Rholl D, Kumar A, Beacham IR, Schweizer HP. Appl Environ Microbiol. 2008 Feb;74(4):1064-75. Epub 2007 Dec 21. 10.1128/AEM.02430-07 PubMed 18156318