|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64963||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Backbone manufacturerHerbert Schweizer
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Growth instructionsalso ampicillin resistant
Copy numberHigh Copy
Gene/Insert namelux operon
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
GenBank Accession # AY962893
Compared to the Genbank reference sequence AY962893, Addgene's sequencing results found a A85R mutation in luxB. The effect of this difference is unknown. Note that Addgene has not sequenced the entire luxCDABE fusion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC18-mini-Tn7T-Gm-lux was a gift from Herbert Schweizer (Addgene plasmid # 64963 ; http://n2t.net/addgene:64963 ; RRID:Addgene_64963)
For your References section:A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923